解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90422-4
更新日期:1989-01-01 00:00:00
abstract::Monoclonal antibodies to the matrix or E1 glycoprotein of mouse hepatitis virus (MHV) were tested for their ability to protect mice from a normally lethal challenge of MHV-4. Four antibodies were tested, and two of these, J.1.3 and J.3.9, were protective. Protection did not correlate with virus neutralization in vitro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90415-7
更新日期:1989-01-01 00:00:00
abstract::The location of the RNA start point of in vivo Q-activated late gene RNA of bacteriophage lambda has been determined to be identical to the start point of in vitro 6 S RNA. The 6 S RNA is made early in infection and is efficiently antiterminated by Q. Two RNase III cut sites are located within Q-dependent RNA sequence...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::The 30K protein of tobacco mosaic virus was recently confirmed to be involved in cell-to-cell movement of the virus. To characterize common structural features of the 30K protein, the nucleotide sequence of the 30K protein gene of cucumber green mottle mosaic virus (CGMMV, a member of the tobamoviruses) RNA has been d...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::The genome of the Chilo iridescent virus (CIV) was analyzed for existence of repetitive DNA sequences by DNA-DNA hybridization using a defined and complete gene library of the viral genome (209 kbp) and by heteroduplex mapping. These experiments revealed the presence of repetitive DNA elements in the CIV genome, which...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90085-2
更新日期:1988-11-01 00:00:00
abstract::Five plaque-forming viruses (Pbi viruses) of the unicellular, eukaryotic, exsymbiotic Chlorella-like green alga strain Pbi were isolated from fresh water collected in Germany. The viruses were compared to two previously characterized plaque-forming viruses (NC64A viruses) of Chlorella strain NC64A. The Pbi viruses do ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90063-3
更新日期:1988-11-01 00:00:00
abstract::The circular single-stranded viral DNA (ssDNA) of filamentous phage is oriented in the virus with a hairpin forming sequence called mos at the leading end of the virion-the end that emerges from the cell first. In the experiments that originally defined mos it was also found to enhance virion production 100-fold, thou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90066-9
更新日期:1988-11-01 00:00:00
abstract::We have examined the expression of duck hepatitis B virus (DHBV)-associated proteins in experimentally infected ducks by an immunoblot (Western) method. The DHBV-related core protein, C protein, was identified at the position of 35,000 Da (P35). Pre-S proteins were recognized as two major bands (P37 and P28), the form...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90056-6
更新日期:1988-11-01 00:00:00
abstract::The 130-kDa bovine herpesvirus-1 (BHV-1) glycoprotein GVP 6 was found to cross-react immunologically with the herpes simplex glycoprotein gB. Antibodies in polyclonal serum against gB immunoprecipitated GVP 6 and its cleavage products from a lysate of BHV-1-infected cells. Conversely, polyclonal serum against GVP 6 pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90525-9
更新日期:1988-10-01 00:00:00
abstract::The mutations in two DNA-negative ts mutants of herpes simplex virus type 1 (HSV-1), tsK13 and tsM19, have been previously mapped to a 2.0-kb fragment (coordinates 0.095-0.108) at the left end of the genome (S. Weller, D. Aschman, W. Sacks, D. Coen, and P. Schaffer, 1983, Virology 130, 290-305). Sequence analysis of t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90507-7
更新日期:1988-10-01 00:00:00
abstract::The 64K surface protein of budded Autographa californica nuclear polyhedrosis virus (AcMNPV BV) is known to play a role in the functional entry of AcMNPV BV into Spodoptera frugiperda IPLB-SF-21 cells by adsorptive endocytosis. AcV1, a neutralizing monoclonal antibody, reacts with the 64K protein and in doing so preve...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90176-6
更新日期:1988-09-01 00:00:00
abstract::We have previously reported that a novel herpes simplex virus RNA transcript partially overlapping the gene encoding ICPO and expressed from the opposite DNA strand is abundant in sensory neurons of mice harboring a latent infection [J.G. Stevens, E.K. Wagner, G.B. Devi-Rao, M.L. Cook, and L.T. Feldman, Science 235, 1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90169-9
更新日期:1988-09-01 00:00:00
abstract::The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90155-9
更新日期:1988-09-01 00:00:00
abstract::Influenza viruses grown in embryonated chicken eggs frequently possess antigenically distinguishable hemagglutinin (HA) compared to virus from the same source grown in mammalian cell culture. To further investigate the extent of variation among viruses from an individual, viruses were isolated from throat washes colle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90588-0
更新日期:1988-08-01 00:00:00
abstract::Avarol is a sesquiterpenoid hydroquinone, which displays no inhibitory potencies on mammalian DNA polymerases alpha, beta, and gamma, on mammalian RNA polymerases I, II, and III, or on reverse transcriptases from Moloney murine leukemia virus (Mo-MuLV) and from HIV. For a further elucidation of the antiviral effect of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90596-x
更新日期:1988-08-01 00:00:00
abstract::The nucleotide sequence of the portion of naturally occurring defective EBV (HR-1) DNA (designated het DNA) which is responsible for disruption of EBV latency has been determined and compared with the regions of the standard HR-1 viral genome from which it was derived. This rearranged 2.7-kbp DNA fragment represented ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90599-5
更新日期:1988-08-01 00:00:00
abstract::The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,72...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90677-0
更新日期:1988-07-01 00:00:00
abstract::The close association between human papillomavirus type 16 and cervical cancer implies some role for the virus in the development of this cancer. It has been demonstrated that HPV-16 DNA sequences in the presence of activated ras are capable of transforming primary baby rat kidney cells. This communication describes t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90695-2
更新日期:1988-07-01 00:00:00
abstract::Human papillomavirus type 6 (HPV 6) DNA was detected in an episomal form in DNA extracted from an invasive squamous carcinoma of the vulva. The viral DNA, designated HPV6-T70, was molecularly cloned. Restriction analysis of the HPV6-T70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90676-9
更新日期:1988-07-01 00:00:00
abstract::Sequences critical for the activity of the measles virus (MV) RNA polymerase in transcription and replication were analyzed using a MV genomic cDNA library containing overlapping clones encompassing the entire MV genome. Clones corresponding to the 3' and 5' ends of the MV genome were identified and sequenced, and the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90564-8
更新日期:1988-06-01 00:00:00
abstract::Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90555-7
更新日期:1988-06-01 00:00:00
abstract::A method was devised which allows the easy detection of mutations within the ner gene of Mu DNA. This method is based upon the observation that a transcriptional gene A-galK fusion containing the complete ner gene and the cts62 allele does not express the galK gene in an Escherichia coli strain lacking functional inte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90621-6
更新日期:1988-05-01 00:00:00
abstract::Three different protein molecules copurify with T4 tail tubes after the tubes are released from the baseplate by guanidine hydrochloride treatment. These tube-associated proteins (TAPs) are the products of genes 29, 48, and 54. To further investigate the structural roles that these proteins may play in T4 tail assembl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90622-8
更新日期:1988-05-01 00:00:00
abstract::We have studied somatically murine leukemia proviral integrations in primary N-methyl-N-nitrosourea (MNU)-induced thymic lymphomas of AKR/J mice. The majority of MNU-induced lymphomas contain newly acquired murine leukemia proviral sequences. In contrast to spontaneous AKR/J lymphomas which contain multiple integratio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90638-1
更新日期:1988-05-01 00:00:00
abstract::Early region 1 (E1) of the human adenoviruses has many intriguing properties which have prompted numerous mutational studies to help delineate and characterize the domains responsible for these functions. In mutational analyses being done currently, the E1 region is usually cloned into a bacterial plasmid where it is ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90302-9
更新日期:1988-04-01 00:00:00
abstract::The location of the cistron encoding the genome-linked protein (VPg) in the potyvirus tobacco vein mottling virus (TVMV) was investigated. Precipitation of 125I-labeled VPg with anti-tobacco etch virus 49K nuclear inclusion protein antiserum (which reacts with the NIa nuclear inclusion protein of TVMV) indicated that ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90307-8
更新日期:1988-04-01 00:00:00
abstract::The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90275-9
更新日期:1988-04-01 00:00:00
abstract::We have characterized capsids made by seven temperature-sensitive (ts) mutants of HSV-1 previously shown to be defective in viral DNA processing and packaging at the nonpermissive temperature (NPT). The empty capsids isolated from mutant-infected cells at the NPT were devoid of DNA, cosedimented in sucrose with wt B c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90288-7
更新日期:1988-04-01 00:00:00
abstract::The major DNA-binding protein or infected cell protein 8 (ICP8) encoded by herpes simplex virus exhibits multiple interactions with the cell nucleus in that it interacts with the host cell nuclear matrix and viral DNA molecules as sequential stages in its maturational process (M. P. Quinlan, L. B. Chen, and D. M. Knip...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90272-3
更新日期:1988-04-01 00:00:00
abstract::The adenovirus (Ad) single-stranded DNA binding protein (DBP) is a multifunctional protein. It is thought to consist of two domains, the amino-terminal domain involved in host-range determination and the carboxyl-terminal domain functioning in DNA replication and DNA binding. We have determined the nucleotide sequence...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90227-9
更新日期:1988-03-01 00:00:00
abstract::The molecular mechanism(s) by which human immunodeficiency virus (HIV-1) injures a T-cell line was studied. A pathological role for viral env proteins, which are inserted into the plasma membrane, has been previously demonstrated for HIV as well as other retroviruses which are cytopathic. We therefore initiated studie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90232-2
更新日期:1988-03-01 00:00:00
abstract::Antibodies against the L protein of Sendai virus were made by immunizing rabbits with a synthetic peptide representing a carboxyl-terminal region of the protein predicted from the base sequence of its gene. These antibodies were used to localize the L protein in viral nucleocapsids by electron microscopy. Immunogold l...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90257-7
更新日期:1988-03-01 00:00:00
abstract::A nonhemagglutinating mutant of a 1978 isolate of canine parvovirus (CPV) was derived after repeated passages in the NLFK feline kidney cell line. The mutant CPV was antigenically indistinguishable from wild-type virus when tested with 82 monoclonal antibodies, and it replicated in cat and dog cell lines in culture. S...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90255-3
更新日期:1988-03-01 00:00:00
abstract::The nucleotide sequence of two variants of coconut tinangaja viroid (CTiV) were obtained. Both sequence variants are 254 nucleotide residues in size but differ in sequence at two positions. In comparisons with other viroids, the sequences and proposed secondary structure of CTiV show most homology with the 246 nucleot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90497-7
更新日期:1988-02-01 00:00:00
abstract::We describe here the transduction of murine hematopoietic progenitor cells with the dominant selectable neomycin drug-resistance (Neo) gene using a recombinant adeno-associated virus (AAV) vector. Successful transformation of progenitor cells to drug resistance was determined to be approximately 1.5% by colony formati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90491-6
更新日期:1988-02-01 00:00:00
abstract::The 5'-terminal noncoding region sequences were determined for the genome RNAs of seven strains of St. Louis encephalitis virus (SLEV) and one strain of West Nile virus (WNV) using a single synthetic cDNA primer complementary to the 5'-terminus of the coding region of a strain of WNV RNA. The 5'-terminal sequences obt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90468-0
更新日期:1988-02-01 00:00:00
abstract::Following infection with murine cytomegalovirus (MCMV), the termini of the linear double-stranded DNA genome fuse to form circular or concatemeric forms which serve as replicative intermediates. To investigate the mechanisms involved in the generation and cleavage of the intracellular concatenates, we have used restri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90398-4
更新日期:1988-01-01 00:00:00
abstract::DNA obtained from New Zealand white rabbit oral papillomas was analyzed for the presence of papillomavirus DNA. The viral genome was cloned as three separate subclones, which were each mapped and oriented with respect to one another. Comparisons with other papillomavirus DNAs by Southern blot hybridization under vario...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90411-4
更新日期:1988-01-01 00:00:00
abstract::Influenza viruses of the H3N2 subtype similar to Aichi/2/68 and Victoria/3/75 persist in pigs many years after their antigenic counterparts have disappeared from humans (Shortridge et al. (1977). Science 19, 1454-1455). To provide information on the mechanism of conservation of these influenza viruses in pigs, the hem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90405-9
更新日期:1988-01-01 00:00:00